Anti-pan-PMCA; clone 5F10

Code: MABN1802 D2-231

Application

Anti-pan-PMCA, clone 5F10, Cat. No. MABN1802, is a mouse monoclonal antibody that targets pan-Plasma membrane calcium-transporting ATPase (PMCA) and has been test...


read more

Your Price
€457.00 EACH
€562.11 inc. VAT

Application

Anti-pan-PMCA, clone 5F10, Cat. No. MABN1802, is a mouse monoclonal antibody that targets pan-Plasma membrane calcium-transporting ATPase (PMCA) and has been tested in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Research CategoryNeuroscience

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected pan-PMCA in human pancreas and human kidney tissue.

Immunocytochemistry Analysis: A representative lot detected pan-PMCA in Immunocytochemistry applications (Paszty, K., et. al. (2005). Biochem J. 391(Pt 3):687-92; DeMarco, S.J., et. al. (2002) J Biol Chem. 277(12):10506-11).

Immunoprecipitation Analysis: A representative lot immunoprecipitated pan-PMCA in Immunoprecipitation applications (DeMarco, S.J., et. al. (2002) J Biol Chem. 277(12):10506-11).

Western Blotting Analysis: A representative lot detected pan-PMCA in Western Blotting applications (Paszty, K., et. al. (2005). Biochem J. 391(Pt 3):687-92; Caride, A.J., et. al. (1996). Biochem J. 316 ( Pt 1):353-9).

ELISA Analysis: A representative lot detected pan-PMCA in ELISA applications (Caride, A.J., et. al. (1996). Biochem J. 316 ( Pt 1):353-9).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Plasma membrane calcium-transporting ATPase (isoforms 1-4) UniProt: P20020, Q01814, Q16720, P23634; also known as PMCA, Plasma membrane calcium pump) is encoded by the ATP2B1, ATP2B2, ATP2B3, ATP2B4 genes (Gene ID: 490; 491; 492; 493) in human. PMCAs are multi-pass membrane proteins that act as a calcium/calmodulin-regulated and magnesium-dependent enzymes and catalyze the hydrolysis of ATP coupled with the transport of calcium out of the cell. PMCAs serve as the main off switch for calcium signals in all types of cells. Although there are four separate genes that code for PMCAs 1-4, alternative splicing of the primary transcripts further increases the number of PMCA variants to about 20, which differ significantly in their regulatory and kinetic properties. PMCA activity is shown to be regulated at (a) the transcriptional level (b) by calmodulin binding and (c) by their subcellular localization. The localization of different PMCAs is regulated in various complex, cell type-specific ways. PMCA1 is ubiquitously expressed, PMCA2 is mainly expressed in brain cortex, but lower levels are also found in skeletal muscle, heart muscle, stomach, liver, kidney and lung. PMCA3 is highly expressed in the cerebellum, particularly in the presynaptic terminals of parallel fibers-Purkinje neurons. PMCA4 is expressed ubiquitously, however, its tissue distribution varies with its isoforms. (Ref.: Antalffy, G et al. (2013). Biochim. Biophys. Acta 1833(12); 2561-2572; Strehler, EE (2015). Biochem. Biophys. Commun. 460 (1); 26-33)

Immunogen

Purified human erythrocyte calcium ATPase.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected pan-PMCA in human cerebral cortex tissue sections.

Specificity

Clone 5F10 detects all isoforms of Plasma membrane calcium-transporting ATPase (PMCA) in human, mouse, and rat.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

134 to 139 kDa calculated.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
clone5F10, monoclonal
Gene Informationhuman ... ATP2B1(490), ATP2B3(492), ATP2B4(493)
isotypeIgG2aκ
NCBI accession no.NP_068768.2, NP_001001331, NP_001001323.1, NP_001001396.1
packagingantibody small pack of 25 µg
Quality Level100
shipped inambient
species reactivityhuman, chicken, rat
technique(s)immunocytochemistry: suitable, western blot: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, ELISA: suitable
UniProt accession no.P20020, Q01814, Q16720, P23634
This product has met the following criteria to qualify for the following awards:



PROCEED TO CHECKOUT

HAVE AN ACCOUNT? LOGIN


GUEST CHECKOUT

Proceed as a guest. You will have the option to register to access exclusive pricing and stock availability features after checkout.